Immunochemical Studies on Human Plasma Lipoproteins
نویسندگان
چکیده
Low density human plasma lipoproteins S(f) 17+, S(f) 13, and S(f) 6, high density lipoproteins 2 and 3, and a lipoprotein-free plasma protein fraction were isolated from human plasma by ultracentrifugal methods. It was found that human plasma lipoproteins are immunochemically distinct from the non-lipoprotein containing plasma protein fraction. Lipoprotein fractions of a given hydrated density, isolated from different individuals, were found to be immunochemically indistinguishable by qualitative absorption tests. Qualitative antigenic differences were shown to exist between low density lipoproteins and high density lipoproteins. Quantitative precipitin reactions showed that low density lipoproteins S(f) 6 and S(f) 13 were immunochemically very similar. However, they differed with respect to the amount of antigen nitrogen required for maximum precipitation. Agar diffusion analyses were performed; the results suggest heterogeneity of lipoproteins by this criterion.
منابع مشابه
Studies of the proteins in human plasma very low density lipoproteins.
The protein components of human plasma very low density lipoproteins (Sf > 20) were studied following partial and total delipidation. After the neutral lipids were extracted with heptane, the resulting phospholipid-protein complexes contained at least one immunochemical reactant different from the major apoproteins of high density and low density lipoproteins. Purification required total delipi...
متن کاملThe lipoproteins and lipid transport in abetalipoproteinemia.
The disease abetalipoproteinemia results in intriguing disturbances of lipid transport (2, 3). Included among its manifestations are an inability to form chylomicrons (4) and the lowest concentrations of plasma lipids detected in any human disorder. In this familial syndrome it is possible that the elaboration of beta lipoprotein, or more strictly its beta or B apoprotein, is primarily affected...
متن کاملImmunochemical heterogeneity of human plasma apolipoprotein B. I. Apolipoprotein B binding of mouse hybridoma antibodies.
Apolipoprotein B obtained from each of the major density classes of human plasma lipoproteins was investigated immunochemically. Eleven different mouse hybridoma cell lines were generated and selected on the basis of their ability to secrete antibodies that were bound by either very low density or intermediate density lipoproteins. Two of these antibodies appeared to be specific for complex epi...
متن کاملThe synthesis of plasma lipoprotein by rat liver.
The immunochemical technique, first employed in the study of albumin synthesis in chicken liver slices by Peters and Anfmsen (3), has recently been successfully used with rat liver slices by Marsh and Drabkin (4) as well as by Campbell and Stone (5). The present work extends this technique to the problem of the biosynthesis of plasma lipoprotein by rat liver. The liver has been shown to be the ...
متن کاملCanine lipoproteins and atherosclerosis. I. Isolation and characterization of plasma lipoproteins from control dogs.
Canine plasma lipoproteins were fractionated into four distinct classes by ultracentrifugation combined with Geon-Pevikon block electrophoresis and characterized with respect to physical and chemical properties. The distribution of plasma lipids and lipoproteins was quite unlike that in man, the dog having approximately five to six times as much high density as lower density lipoproteins. Despi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 105 شماره
صفحات -
تاریخ انتشار 1957